Difference between revisions of "Part:BBa K4724004:Design"

 
(Source)
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
It's from LSPETase4.
+
It's from LSPET4.
  
 
===References===
 
===References===

Latest revision as of 14:21, 11 October 2023


CBM4-6xHis Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 295
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 216
    Illegal AgeI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

You must use a flexible amino acid chain long enough to link it with protein.


Source

It's from LSPET4.

References