Difference between revisions of "Part:BBa K4623014:Design"
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Latest revision as of 13:51, 11 October 2023
Basic Silinker expression pathway（T7）
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 520
Illegal AgeI site found at 580 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
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Source
The T7 promoter, RBS and T7 terminator were derived from the pETDuet-1 plasmid skeleton. Other parts were derived from DNA sequences after codon optimization according to the amino acid sequence