Difference between revisions of "Part:BBa K4960016"
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N-peptide that guides payload loading
 
N-peptide that guides payload loading
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
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===Profile===
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Name: Pdp1-NTD<br>
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Base Pairs: 202bp<br>
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Origin: Photorhabdus<br>
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Properties: N-peptide that guides payload loading<br>
 
===Usage and Biology===
 
===Usage and Biology===
 
PVCs are eCISs produced by members of the genus Photorhabdus, which exist as endosymbionts within entomopathogenic nematodes. As a toxin, PVC targets the host insect, delivering the PVC payload Pdp1 and Pnf to kill the insect[1].<br>
 
PVCs are eCISs produced by members of the genus Photorhabdus, which exist as endosymbionts within entomopathogenic nematodes. As a toxin, PVC targets the host insect, delivering the PVC payload Pdp1 and Pnf to kill the insect[1].<br>
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N-terminal packaging domains of Pdp1 and Pnf are necessary and sufficient for loading of these proteins into a PVC particle.<br>
 
N-terminal packaging domains of Pdp1 and Pnf are necessary and sufficient for loading of these proteins into a PVC particle.<br>
 
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===Sequencing===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960016 SequenceAndFeatures</partinfo>
  
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===References===
 
===References===
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[1] Yang, G., Dowling, A. J., Gerike, U., ffrench-Constant, R. H. & Waterfield, N. R.  
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[1] Yang, G., Dowling, A. J., Gerike, U., ffrench-Constant, R. H. & Waterfield, N. R. Photorhabdus virulence cassettes confer injectable insecticidal activity against the wax moth. J. Bacteriol. 188, 2254–2261 (2006).<br>
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Photorhabdus virulence cassettes confer injectable insecticidal activity against the wax  
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−
moth. J. Bacteriol. 188, 2254–2261 (2006).<br>
+
 
[2] Wang X, Cheng J, Shen J, Liu L, Li N, Gao N, Jiang F, Jin Q. Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica. Sci China Life Sci. 2022 Mar;65(3):618-630.<br>
 
[2] Wang X, Cheng J, Shen J, Liu L, Li N, Gao N, Jiang F, Jin Q. Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica. Sci China Life Sci. 2022 Mar;65(3):618-630.<br>
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[3] Kreitz, J., Friedrich, M.J., Guru, A. et al. Programmable protein delivery with a bacterial contractile injection system. Nature 616, 357–364 (2023).<br>
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[3] Kreitz, J., Friedrich, M.J., Guru, A. et al. Programmable protein delivery with a bacterial contractile injection system. Nature 616, 357–364 (2023).<br>

Revision as of 13:21, 11 October 2023


Pdp1-NTD
N-peptide that guides payload loading

Profile

Name: Pdp1-NTD
Base Pairs: 202bp
Origin: Photorhabdus
Properties: N-peptide that guides payload loading

Usage and Biology

PVCs are eCISs produced by members of the genus Photorhabdus, which exist as endosymbionts within entomopathogenic nematodes. As a toxin, PVC targets the host insect, delivering the PVC payload Pdp1 and Pnf to kill the insect[1].
The toxin is responsible for the cytotoxicity towardsthe eukaryotic cells on the basis of its dNTP pyrophosphataseactivity. [2]
Pdp1(Photorhabdus dNTP pyrophosphatase 1) is a widespread dual-localized pyrophosphatase toxin ,containing a predicted N-terminal packaging domain. The N-terminal domain (NTD) of Pdp1 is likely disordered as it exhibits a low pLDDT score in AlphaFold (it appears as a long disordered extension in the protein’s predicted structure) as well as a high PONDR VSL2 score49. This N-terminal disordered region could serve as a “packaging domain”—a molecular identifier to assist the PVCs loading machinery in identifying and loading the proper payloads [3].
N-terminal packaging domains of Pdp1 and Pnf are necessary and sufficient for loading of these proteins into a PVC particle.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Yang, G., Dowling, A. J., Gerike, U., ffrench-Constant, R. H. & Waterfield, N. R. Photorhabdus virulence cassettes confer injectable insecticidal activity against the wax moth. J. Bacteriol. 188, 2254–2261 (2006).
[2] Wang X, Cheng J, Shen J, Liu L, Li N, Gao N, Jiang F, Jin Q. Characterization of Photorhabdus Virulence Cassette as a causative agent in the emerging pathogen Photorhabdus asymbiotica. Sci China Life Sci. 2022 Mar;65(3):618-630.
[3] Kreitz, J., Friedrich, M.J., Guru, A. et al. Programmable protein delivery with a bacterial contractile injection system. Nature 616, 357–364 (2023).