Difference between revisions of "Part:BBa K4779003:Design"

 
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===Source===
 
===Source===
  
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CMPSpro is a new composite part (BBa_K4779003). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1(BBa_K1346004), the promoter of prm1 pro(BBa_K3384313)，green fluorescent protein (GFP) (BBa_K2176000), Ste5ΔN-CTM(BBa_K3384315) and the terminator CYC1(BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.
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CMPSpro is a new composite part (BBa_K4779003). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1(BBa_K1346004), the promoter of prm1 pro(BBa_K3384313)，green fluorescent protein (GFP) (BBa_K3112009), Ste5ΔN-CTM(BBa_K3384315) and the terminator CYC1(BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.
  
 
===References===
 
===References===

Latest revision as of 13:21, 11 October 2023


CMPS Pro:A yeast copper-induced reporter pathway with Positive feedback loop with prm1pro promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3138
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 4393
    Illegal SpeI site found at 4939
    Illegal PstI site found at 49
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3138
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 4393
    Illegal SpeI site found at 4939
    Illegal PstI site found at 49
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3138
    Illegal BglII site found at 55
    Illegal BglII site found at 3586
    Illegal BglII site found at 4894
    Illegal BamHI site found at 510
    Illegal XhoI site found at 8
    Illegal XhoI site found at 4831
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3138
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 4393
    Illegal SpeI site found at 4939
    Illegal PstI site found at 49
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3138
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 4393
    Illegal SpeI site found at 4939
    Illegal PstI site found at 49
    Illegal NgoMIV site found at 36
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2694
    Illegal SapI site found at 529


Design Notes

When designing the sequence in detail, one of the key considerations is how to convert copper ion signals into pheromone signals more efficiently and sensitively. Drawing from the insights of the second DBTL, specifically in the context of CMPS (BBa_K4779002) with its incorporated positive feedback loop utilizing Ste5ΔN-CTM, we replaced the original pprm1 with the more sensitive pprm1 Pro promoter for GFP expression. This strategic modification significantly amplifies the sensitivity in detecting pheromone signals, resulting in the creation of CMPS Pro (BBa_K4779003). This is the third round DBTL.


Source

CMPSpro is a new composite part (BBa_K4779003). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1(BBa_K1346004), the promoter of prm1 pro(BBa_K3384313)，green fluorescent protein (GFP) (BBa_K3112009), Ste5ΔN-CTM(BBa_K3384315) and the terminator CYC1(BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.

References