Difference between revisions of "Part:BBa K4779002:Design"

 
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===Source===
 
===Source===
  
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CMPS is a new composite part (BBa_K4779002). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1 (BBa_K1346004), green fluorescent protein (GFP) (BBa_K2176000), Ste5ΔN-CTM (BBa_K3384315) and the terminator CYC1 (BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.
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CMPS is a new composite part (BBa_K4779002). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1 (BBa_K1346004), green fluorescent protein (GFP) (BBa_K3112009), Ste5ΔN-CTM (BBa_K3384315) and the terminator CYC1 (BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.
  
 
===References===
 
===References===

Latest revision as of 13:20, 11 October 2023


CMPS:A yeast copper-induced reporter pathway with Positive feedback loop in with prm1 promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2657
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 3912
    Illegal SpeI site found at 4458
    Illegal PstI site found at 49
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2657
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 3912
    Illegal SpeI site found at 4458
    Illegal PstI site found at 49
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2657
    Illegal BglII site found at 55
    Illegal BglII site found at 3105
    Illegal BglII site found at 4413
    Illegal BamHI site found at 510
    Illegal XhoI site found at 8
    Illegal XhoI site found at 4350
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2657
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 3912
    Illegal SpeI site found at 4458
    Illegal PstI site found at 49
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2657
    Illegal XbaI site found at 279
    Illegal XbaI site found at 517
    Illegal SpeI site found at 343
    Illegal SpeI site found at 523
    Illegal SpeI site found at 3912
    Illegal SpeI site found at 4458
    Illegal PstI site found at 49
    Illegal NgoMIV site found at 36
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2213
    Illegal SapI site found at 529


Design Notes

When meticulously designing sequences, one of the critical considerations is how to enhance the sensitivity and efficiency of converting copper ion signals into pheromone signals. According to the literature, Ste5ΔN-CTM has been reported to anchor Ste5 constitutively to the plasma membrane, thereby maintaining the activation of the MAPK pathway. Therefore, in this round DBTL, we utilized Ste5ΔN-CTM to construct a positive feedback loop, where the pheromone-inducible promoter pprm1 controls the expression of Ste5ΔN-CTM protein. This activation enhances the amplification effect of the MAPK pathway, further elevating the sensitivity in detecting pheromone which was produced by pCUP1.


Source

CMPS is a new composite part (BBa_K4779002). We obtained the gene sequences of the promoter of Cup1 (BBa_K945002), MFα2 (BBa_K110005), the promoter of prm1 (BBa_K1346004), green fluorescent protein (GFP) (BBa_K3112009), Ste5ΔN-CTM (BBa_K3384315) and the terminator CYC1 (BBa_K4278703) from IGEM, and sent them to GENEWIZ (Suzhou Genewiz Biotechnology Co., Ltd.) for synthesis.

References