Difference between revisions of "Part:BBa K4879006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The expression cassette contained upstream and downstream sequences homologous to the faa1 gene locus, in order to delete the gene by homologous recombination. | |
===Source=== | ===Source=== | ||
| − | + | The expression construct was synthesised by IDT. | |
| − | + | ||
===References=== | ===References=== | ||
Revision as of 13:16, 11 October 2023
JcFATA expression construct
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1642
Illegal suffix found in sequence at 1912
Illegal EcoRI site found at 1906
Illegal PstI site found at 862
Illegal PstI site found at 1469 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1642
Illegal EcoRI site found at 1906
Illegal SpeI site found at 1913
Illegal PstI site found at 862
Illegal PstI site found at 1469
Illegal PstI site found at 1927
Illegal NotI site found at 1648
Illegal NotI site found at 1920 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1642
Illegal EcoRI site found at 1906
Illegal BglII site found at 1311 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1642
Illegal suffix found in sequence at 1913
Illegal EcoRI site found at 1906
Illegal PstI site found at 862
Illegal PstI site found at 1469 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1642
Illegal EcoRI site found at 1906
Illegal XbaI site found at 1657
Illegal SpeI site found at 1913
Illegal PstI site found at 862
Illegal PstI site found at 1469
Illegal PstI site found at 1927 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The expression cassette contained upstream and downstream sequences homologous to the faa1 gene locus, in order to delete the gene by homologous recombination.
Source
The expression construct was synthesised by IDT.