Difference between revisions of "Part:BBa K4960014"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960014 short</partinfo>
 
<partinfo>BBa_K4960014 short</partinfo>
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===Properties===
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===Profile===
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Name: PVC15<br>
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Base Pairs: 2097bp<br>
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Origin: Photorhabdus<br>
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Properties: Pvc15 functions as tape measure protein and ATPase.<br>
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
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<html>
  
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PVC15 functions as tape measure protein and ATPase
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
  
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</figure>
  
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<!-- Add more about the biology of this part here
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</html>
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===Usage and Biology===
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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Being present at very low levels in the PVC dsyringes
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<br>
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sample.
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Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase.
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Could be localized in the map and were not modeled.
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With putative functions as tape measure protein and ATPase, are likely not present in the mature PVC syringes.
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Being absent in CISs like phage T4 or R-type pyocins.
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===References===
 
===References===
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[1] J. Kreitz et al., “Programmable protein delivery with a bacterial contractile injection system,” Nature, vol. 616, no. 7956, pp. 357–364, Apr. 2023, doi: 10.1038/s41586-023-05870-7.<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2] F. Jiang et al., “Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System,” Cell, vol. 177, no. 2, pp. 370-383.e15, Apr. 2019, doi: 10.1016/j.cell.2019.02.020.
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Revision as of 12:57, 11 October 2023


pvc15

Profile

Name: PVC15
Base Pairs: 2097bp
Origin: Photorhabdus
Properties: Pvc15 functions as tape measure protein and ATPase.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

Pvc15 is present at very low levels in the PVC syringes sample and is absent in CISs like phage T4 or R-type pyocins.According to previous studies,it possibly functions as tape measure protein and ATPase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 87
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 618
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 1056
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.