Difference between revisions of "Part:BBa K4880015:Design"

 
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===Design Notes===
 
===Design Notes===
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In our design, the expression of santalene and santalol synthases are controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.
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===Source===
 
===Source===

Latest revision as of 12:47, 11 October 2023


Ptrc-theo-SaSS-CYP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3105
    Illegal PstI site found at 1023
    Illegal PstI site found at 2853
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3105
    Illegal PstI site found at 1023
    Illegal PstI site found at 2853
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3105
    Illegal XhoI site found at 2441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3105
    Illegal PstI site found at 1023
    Illegal PstI site found at 2853
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3105
    Illegal PstI site found at 1023
    Illegal PstI site found at 2853
    Illegal NgoMIV site found at 1942
    Illegal NgoMIV site found at 2254
    Illegal AgeI site found at 55
    Illegal AgeI site found at 984
    Illegal AgeI site found at 1623
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1418
    Illegal BsaI site found at 2553


Design Notes

In our design, the expression of santalene and santalol synthases are controlled by IPTG and theophylline. Transcription is regulated by IPTG and translation initiates only when there is theophylline present.

Source

This composite part combines BBa_K3930029, BBa_K4880001 and BBa_K4880014.

References