Difference between revisions of "Part:BBa K4879000:Design"
Sauron 3791 (Talk | contribs) |
Sauron 3791 (Talk | contribs) |
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The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner. | The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner. | ||
| − | https://www.ncbi.nlm.nih.gov/gene/ | + | https://www.ncbi.nlm.nih.gov/gene/105644367 |
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===References=== | ===References=== | ||
Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209 | Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209 | ||
Latest revision as of 12:42, 11 October 2023
JcFATA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 331
Illegal PstI site found at 938 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 331
Illegal PstI site found at 938 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 780
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 331
Illegal PstI site found at 938 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 331
Illegal PstI site found at 938 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.
Source
The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.
https://www.ncbi.nlm.nih.gov/gene/105644367
References
Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209