Difference between revisions of "Part:BBa K4623003"

Revision as of 12:33, 11 October 2023

TrwC, a protein linker to covalently link ssDNA 5' end

TrwC is a type of relaxase, which participates in bacterial DNA binding and horizontal transfer of genomic material between bacteria ^[1]. In vitro, it can specifically recognize the nic sequence (GGTGCGTATTGTCT^ATAGC) on ssDNA, causing it to break, and covalently bind to the ssDNA.

Therefore, TrwC relaxase possesses sequence specificity, orthogonality, and the ability to form a covalent bond with standard oligonucleotides. This provides a potential method in our project design that may be superior to existing protein-bound DNA nanostructures. The linkage of TrwC with ssDNA in vitro depends on Mg²⁺, so the reaction can be initiated by adding Mg²⁺ in the mixed system. Conversely, adding EDTA can terminate the reaction and yield DNA-protein complexes ^[2].

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 286
Illegal PstI site found at 604
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 286
Illegal PstI site found at 604
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 286
Illegal PstI site found at 604
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 286
Illegal PstI site found at 604
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 409
Illegal SapI.rc site found at 124

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 <partinfo>BBa_K4623003 short</partinfo>
-TrwC is a type of relaxase, which participates in bacterial DNA binding and horizontal transfer of genomic material between bacteria&#8203; [1]&#8203;. In vitro, it can specifically recognize the nic sequence (GGTGCGTATTGTCT^ATAGC) on ssDNA, causing it to break, and covalently bind to the ssDNA.
+TrwC is a type of relaxase, which participates in bacterial DNA binding and horizontal transfer of genomic material between bacteria&#8203; <sup>[1]</sup>&#8203;. In vitro, it can specifically recognize the nic sequence (GGTGCGTATTGTCT^ATAGC) on ssDNA, causing it to break, and covalently bind to the ssDNA.
-Therefore, TrwC relaxase possesses sequence specificity, orthogonality, and the ability to form a covalent bond with standard oligonucleotides. This provides a potential method in our project design that may be superior to existing protein-bound DNA nanostructures. The linkage of TrwC with ssDNA in vitro depends on Mg2+, so the reaction can be initiated by adding Mg2+ in the mixed system. Conversely, adding EDTA can terminate the reaction and yield DNA-protein complexes [&#8203;2]&#8203;.
+Therefore, TrwC relaxase possesses sequence specificity, orthogonality, and the ability to form a covalent bond with standard oligonucleotides. This provides a potential method in our project design that may be superior to existing protein-bound DNA nanostructures. The linkage of TrwC with ssDNA in vitro depends on Mg<sup>2+</sup>, so the reaction can be initiated by adding Mg<sup>2+</sup> in the mixed system. Conversely, adding EDTA can terminate the reaction and yield DNA-protein complexes <sup>[2]</sup>&#8203;.
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