Difference between revisions of "Part:BBa K4879005:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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-
+
The coding sequence has been codon-optimized for <i>Y. lipolytica</i> using SnapGene. It also has silent mutations to make the part Type II Standard compatible.
  
  
  
 
===Source===
 
===Source===
 +
The part had been synthesized by IDT, iGEM's DNA synthesis partner.
  
−
-
+
The cDNA sequence was taken from the supplementary information provided in the article attached in the references.
  
 
===References===
 
===References===

Revision as of 12:30, 11 October 2023


Yarrowia lipolytica ScRAD52 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 403
    Illegal BglII site found at 533
    Illegal XhoI site found at 1223
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1207
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by IDT, iGEM's DNA synthesis partner.

The cDNA sequence was taken from the supplementary information provided in the article attached in the references.

References