Difference between revisions of "Part:BBa K4879003:Design"
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===Source=== | ===Source=== | ||
− | The part had been synthesized by | + | The part had been synthesized by IDT, iGEM's DNA synthesis partner. |
The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar. | The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar. | ||
===References=== | ===References=== |
Latest revision as of 12:28, 11 October 2023
Yarrowia lipolytica HygR marker
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 153
Illegal EcoRI site found at 247 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 153
Illegal EcoRI site found at 247 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 153
Illegal EcoRI site found at 247 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 153
Illegal EcoRI site found at 247 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 153
Illegal EcoRI site found at 247 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.
Source
The part had been synthesized by IDT, iGEM's DNA synthesis partner.
The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.