Difference between revisions of "Part:BBa K4879004:Design"
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.
+
The part had been synthesized by IDT, iGEM's DNA synthesis partner.
  
 
The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.
 
The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.
  
 
===References===
 
===References===

Revision as of 12:28, 11 October 2023


Yarrowia lipolytica guaB marker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal BamHI site found at 1081
    Illegal XhoI site found at 851
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 565
    Illegal EcoRI site found at 1019
    Illegal PstI site found at 878
    Illegal NgoMIV site found at 868
    Illegal NgoMIV site found at 1093
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by IDT, iGEM's DNA synthesis partner.

The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.

References