Difference between revisions of "Part:BBa K4879003:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
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-
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The coding sequence has been codon-optimized for <i>Y. lipolytica</i> using SnapGene. It also has silent mutations to make the part Type II Standard compatible.
  
  
  
 
===Source===
 
===Source===
 +
The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.
  
−
-
+
The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.
  
 
===References===
 
===References===

Revision as of 12:24, 11 October 2023


Yarrowia lipolytica HygR marker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 153
    Illegal EcoRI site found at 247
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.


Source

The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.

The DNA sequence, before optimization, was provided by our secondary PI Dr. Mridula Nambiar.

References