Difference between revisions of "Part:BBa K4879002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | - | + | The coding sequence has been codon-optimized for <i>Y. lipolytica</i> using SnapGene. It also has silent mutations to make the part Type II Standard compatible. |
| − | + | ||
===Source=== | ===Source=== | ||
| + | The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner. | ||
| + | |||
| + | The truncated CvFAP cDNA sequence was provided in the supplementary information of the article attached in the references. | ||
| − | |||
===References=== | ===References=== | ||
| + | Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4 | ||
Latest revision as of 12:21, 11 October 2023
CvFAP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1424
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 259
Illegal PstI site found at 691
Illegal PstI site found at 958
Illegal NgoMIV site found at 1579
Illegal AgeI site found at 1555 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.
Source
The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.
The truncated CvFAP cDNA sequence was provided in the supplementary information of the article attached in the references.
References
Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4