Difference between revisions of "Part:BBa K4879001:Design"

Latest revision as of 12:15, 11 October 2023

JcFATB

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 831
Illegal PstI site found at 50
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 831
Illegal PstI site found at 50
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 831
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 831
Illegal PstI site found at 50
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 831
Illegal PstI site found at 50
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The coding sequence has been codon-optimized for Y. lipolytica using SnapGene. It also has silent mutations to make the part Type II Standard compatible.

Source

The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.

https://www.ncbi.nlm.nih.gov/gene/105639285

References

Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209

@@ Line 7: / Line 7: @@
 ===Design Notes===
--
+The coding sequence has been codon-optimized for <i>Y. lipolytica</i> using SnapGene. It also has silent mutations to make the part Type II Standard compatible.
 ===Source===
+The part had been synthesized by Twist Bioscience, iGEM's DNA synthesis partner.
--
+https://www.ncbi.nlm.nih.gov/gene/105639285
 ===References===
+Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209