Difference between revisions of "Part:BBa K4960004"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K4960004 short</partinfo>
 
<partinfo>BBa_K4960004 short</partinfo>
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===Properties===
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===Profile===
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Name: Pvc4<br>
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Pvc4 is a part of the sheath.
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Base Pairs: 1275 bp<br>
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Origin: Photorhabdus<br>
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Properties: Pvc4 is a part of the sheath.<br>
  
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
 
===Usage and Biology===
 
===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1,Figure2)<br>
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)<br>
 
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<html>
  
 
<figure class="figure">
 
<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-3-4-5-6-7.png" class="figure-img img-fluid rounded"  height="200px">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc1-16.jpg" class="figure-img img-fluid rounded"  height="150px">
  
 
</figure>
 
</figure>
  
 
</html>
 
</html>
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Figure1:PVC illustrations<br>
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Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).<br>
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Figure2:Payload illustrations<br>
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<br>
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The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath.  
 
The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath.  
 
Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated.
 
Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated.
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In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12.(Figure3)
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In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12.
  
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<figure class="figure">
 
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc4.png" class="figure-img img-fluid rounded"  height="200px">
 
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc-2-4.png" class="figure-img img-fluid rounded"  height="200px">
 
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</figure>
 
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</html>
 
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Figure3.The protein pvc4 encodes
 
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K4960004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4960004 SequenceAndFeatures</partinfo>
  
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<partinfo>BBa_K4960004 parameters</partinfo>
 
<partinfo>BBa_K4960004 parameters</partinfo>
 
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===Reference===
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===References===
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;<br>
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. <br>
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21. <br>

Revision as of 12:12, 11 October 2023


pvc4

Profile

Name: Pvc4
Base Pairs: 1275 bp
Origin: Photorhabdus
Properties: Pvc4 is a part of the sheath.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1)

Figure 1. Schematic of the PVCpnf locus. It contains 16 structural and accessory genes , followed by two payload genes (Pdp1 and Pnf, in red) and four putative regulatory genes (in pink).

The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath. Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated. In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;177(2):370-383.e15. doi: 10.1016/j.cell.2019.02.020. Epub 2019 Mar 21.