Difference between revisions of "Part:BBa K4800047"

 
 
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<partinfo>BBa_K4800047 short</partinfo>
 
<partinfo>BBa_K4800047 short</partinfo>
  
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To compare the activity of different transaminases, the DNA fragments of Ptrc-RBS-YahK were amplified and ligated into the plasmids of pTrc99a-GabT.
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We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-GabT.
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<!-- Add more about the biology of this part here

Latest revision as of 12:02, 11 October 2023


pTrc99a-YahK-GabT

We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene GabT to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-GabT.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3624
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4039
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5172
    Illegal BsaI site found at 6240
    Illegal SapI.rc site found at 767