Difference between revisions of "Part:BBa K4879000"

Revision as of 11:31, 11 October 2023

JcFATA

The fatty acyl-ACP thioesterase A, from the plant Jatropha curcas. [1]

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Usage and Biology

Plant acyl-ACP thioesterases are enzymes that play a crucial role in various metabolic processes in plants. They function by catalyzing the hydrolysis of thioester bonds specifically between an acyl group and an acyl carrier protein (ACP). This hydrolysis reaction results in the release of the acyl group from ACP, producing a free fatty acid. Thus, acyl-ACP thioesterases are pivotal in regulating the chain length of fatty acids in de novo fatty acid biosynthesis by cleaving them from ACP.

This regulatory function of thioesterases in fatty acids' chain lengths significantly affects the free fatty acid as well as the triglyceride compositions in plant seed oils, affecting their value in terms of nutrition or biofuel potential. This has made thioesterases a focus in industries, with research aimed at manipulating them to give favourable lipid compositions for further processing.

In Jatropha curcas, fatty acyl-ACP thioesterase A primarily acts on linoleoyl-ACP and oleoyl-ACP to give linoleic acid (C 18:2) and oleic acid (C 18:1). The ability of this enzyme to give unsaturated fatty acids–a feedstock much desired for the production of aviation biofuel– at a rate similar to that of its original organism was one of the reasons we had for its expression in our chassis Yarrowia lipolytica.

Protein Structure of JcFATA

The protein structure of JcFATA, as predicted by AlphaFold

<a> href = "https://alphafold.ebi.ac.uk/entry/A0A0M4BUI7"</a>

Design

The transcriptional unit for JcFATA (BBa_K4879006) is designed with the coding sequence flanked by the TEF1 promoter (BBa_K3629001) upstream and the XPR2 terminator (BBa_K3629004) downstream.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 331
Illegal PstI site found at 938
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 331
Illegal PstI site found at 938
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 780
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 331
Illegal PstI site found at 938
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 331
Illegal PstI site found at 938
1000
COMPATIBLE WITH RFC[1000]

References

1. Liu, Y.; Han, J.; Li, Z.; Jiang, Z.; Luo, L.; Zhang, Y.; Chen, M.; Yang, Y.; Liu, Z. Heterologous Expression of Jatropha curcas Fatty Acyl-ACP Thioesterase A (JcFATA) and B (JcFATB) Affects Fatty Acid Accumulation and Promotes Plant Growth and Development in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 4209. https://doi.org/10.3390/ijms23084209

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 <html><img src="https://static.igem.wiki/teams/4879/wiki/bba-k4879000-jcfata.png"alt ="Protein Structure of JcFATA"width="250"height="250"></html>
 The protein structure of JcFATA, as predicted by AlphaFold
+<a> href = "https://alphafold.ebi.ac.uk/entry/A0A0M4BUI7"</a>
 ===Design===
 The transcriptional unit for JcFATA [https://parts.igem.org/Part:BBa_K4879006 (BBa_K4879006)] is designed with the coding sequence flanked by the TEF1 promoter [https://parts.igem.org/Part:BBa_K3629001 (BBa_K3629001)] upstream and the XPR2 terminator [https://parts.igem.org/Part:BBa_K3629004 (BBa_K3629004)] downstream.