Difference between revisions of "Part:BBa K4800050"
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<partinfo>BBa_K4800050 short</partinfo> | <partinfo>BBa_K4800050 short</partinfo> | ||
− | + | We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SAV2585. | |
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Latest revision as of 11:25, 11 October 2023
pTrc99a-YahK-SAV2585
We linearized the pTrc99a-YahK vector using restriction endonuclease and connected the target gene SAV2585 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK-SAV2585.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 4316
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 4316
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3624
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 4316
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 4316
Illegal NgoMIV site found at 3852
Illegal NgoMIV site found at 4516
Illegal NgoMIV site found at 4545
Illegal NgoMIV site found at 4590
Illegal NgoMIV site found at 5394
Illegal NgoMIV site found at 5508
Illegal AgeI site found at 4201
Illegal AgeI site found at 4252
Illegal AgeI site found at 4327
Illegal AgeI site found at 4441 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5781
Illegal BsaI site found at 6849
Illegal SapI.rc site found at 767