Difference between revisions of "Part:BBa K4613006"
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<partinfo>BBa_K4613006 short</partinfo> | <partinfo>BBa_K4613006 short</partinfo> | ||
− | TraR protein forms a complex with its signaling molecule. This complex binds to the <em> | + | TraR protein forms a complex with its signaling molecule. This complex binds to the <em>P<sub>tra<sub></em> promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II、-35 sequences、-10 sequences and an RBS. |
==== Reference ==== | ==== Reference ==== |
Revision as of 10:58, 11 October 2023
Ptra
TraR protein forms a complex with its signaling molecule. This complex binds to the Ptra</em> promoter, increasing the rate of transcription. Compared with BBa_K553002, we have truncated the sequence to 76 bp, including the tra box II、-35 sequences、-10 sequences and an RBS.
Reference
- White C E, Winans S C.Identification of amino acid residues of the Agrobacterium tumefaciens quorum-sensing regulator TraR that are critical for positive control of transcription[J].Mol Microbiol,2005, 55 (5): 1473-86.
- Fuqua C, Winans S C.Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes[J].J Bacteriol,1996, 178 (2): 435-40.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 14
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]