Difference between revisions of "Part:BBa K4907020"

Latest revision as of 10:38, 11 October 2023

araBAD promoter

Biology

The araBAD promoter is an E. coli promoter that is tightly controlled by inducer: L-arabinose.

Usage and design

We used This part to combine hrpR(BBa_K4907021) and hrpS(BBa_K4907022) to construct the hrp system and obtained the composite part BBa_K4907126, which are assembled on the expression vector pSB1C3(Fig. 1).

Fig. 1 Gene circuits of the composite part BBa_K4907126

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 300
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 239
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 74
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 56

@@ Line 8: / Line 8: @@
 ===Usage and design===
-We used This part to combine hrpR(<partinfo>BBa_K4907021</partinfo>) and hrpS(<partinfo>BBa_K4907022</partinfo>) to construct the <i>hrp</i> system and obtained the composite part <partinfo>BBa_K4907126</partinfo>, which are assembled on the expression vector pSB1C3(Fig. 1).
+We used This part to combine <i>hrpR</i>(<partinfo>BBa_K4907021</partinfo>) and <i>hrpS</i>(<partinfo>BBa_K4907022</partinfo>) to construct the <i>hrp</i> system and obtained the composite part <partinfo>BBa_K4907126</partinfo>, which are assembled on the expression vector pSB1C3(Fig. 1).
 <center><html><img src="https://static.igem.wiki/teams/4907/wiki/parts/haoxihuan/i0500-b0034-hrpr-b0015-arabad-promoter-b0034-hrps-b0015.png" width="700px"></html></center>
 <center><b>Fig. 1 Gene circuits of the composite part BBa_K4907126</b></center>