Difference between revisions of "Part:BBa K4613302"

Revision as of 10:16, 11 October 2023

pET-29a(+)-T3-M-CPA

In order to find an appropriate expression intensity to achieve balance between metabolic burden and detection efficiency, we tried the T7 lac promoter from pET-29a(+). The composite part can be directly imported into pET-29a(+) vector and express T3-M-CPA induced with IPTG.

Fig. 1 (a)The plasmid map of pET29a(+)-T3. (b)SDS-PAGE analysis of the purified protein T3 in E. coli BL21 (DE3) cultured in LB medium express protein for 12 hours at 20℃. Lane M: protein marker. Lanes 1-6: flow through and elution containing 10, 50, 50, 100, 100, 250, 250 mM imidazole, respectively.

Reference

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3. Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
4. Xiong L, Peng M, Zhao M, et al.Truncated Expression of a Carboxypeptidase A from Bovine Improves Its Enzymatic Properties and Detoxification Efficiency of Ochratoxin A[J].Toxins (Basel),2020, 12 (11).

Sequence and Features