Difference between revisions of "Part:BBa K212001"
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In our system, the Tar-EnvZ (or Taz) chimera protein is used to indicate and signal the presence of aspartate as a chemical ligand. The native form of Tar, found in E. coli, has three domains: a periplasmic ligand binding receptor domain, a transmembrane domain, and an intracellular kinase domain. When aspartate binds to the receptor domain, the kinase domain subsequently transduces a message by phosphorylating CheA and CheY, which orients the bacteria's chemotaxis machinery (i.e. flagellae) towards the aspartate source. In the Taz construct, however, the kinase domain is omitted and the receptor and signal transduction domains of Tar are ligated to the kinase domain of EnvZ, natively an osmolarity sensor kinase-phosphotransferase. Through the kinase activity of EnvZ, this chimeric protein phosphorylates transcription factor OmpR, which subsequently activates transcription of the OmpC gene (or whatever is under the OmpC promoter). | In our system, the Tar-EnvZ (or Taz) chimera protein is used to indicate and signal the presence of aspartate as a chemical ligand. The native form of Tar, found in E. coli, has three domains: a periplasmic ligand binding receptor domain, a transmembrane domain, and an intracellular kinase domain. When aspartate binds to the receptor domain, the kinase domain subsequently transduces a message by phosphorylating CheA and CheY, which orients the bacteria's chemotaxis machinery (i.e. flagellae) towards the aspartate source. In the Taz construct, however, the kinase domain is omitted and the receptor and signal transduction domains of Tar are ligated to the kinase domain of EnvZ, natively an osmolarity sensor kinase-phosphotransferase. Through the kinase activity of EnvZ, this chimeric protein phosphorylates transcription factor OmpR, which subsequently activates transcription of the OmpC gene (or whatever is under the OmpC promoter). | ||
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''Adapted from'' '''FIG. 8. A model for signal transduction through the cytoplasmic domain of EnvZ''' from Ref 2. | ''Adapted from'' '''FIG. 8. A model for signal transduction through the cytoplasmic domain of EnvZ''' from Ref 2. | ||
Latest revision as of 01:53, 20 October 2009
Tar-Envz (Taz)
In our system, the Tar-EnvZ (or Taz) chimera protein is used to indicate and signal the presence of aspartate as a chemical ligand. The native form of Tar, found in E. coli, has three domains: a periplasmic ligand binding receptor domain, a transmembrane domain, and an intracellular kinase domain. When aspartate binds to the receptor domain, the kinase domain subsequently transduces a message by phosphorylating CheA and CheY, which orients the bacteria's chemotaxis machinery (i.e. flagellae) towards the aspartate source. In the Taz construct, however, the kinase domain is omitted and the receptor and signal transduction domains of Tar are ligated to the kinase domain of EnvZ, natively an osmolarity sensor kinase-phosphotransferase. Through the kinase activity of EnvZ, this chimeric protein phosphorylates transcription factor OmpR, which subsequently activates transcription of the OmpC gene (or whatever is under the OmpC promoter).
Adapted from FIG. 8. A model for signal transduction through the cytoplasmic domain of EnvZ from Ref 2.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 420
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 420
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 420
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 111