Difference between revisions of "Part:BBa K4879009"

 
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<partinfo>BBa_K4879009 short</partinfo>
 
<partinfo>BBa_K4879009 short</partinfo>
  
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The transcriptional unit for HygR, designed for expression in <i>Y. lipolytica</i> in order to delete the faa1 gene from our chassis.
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===Characterization===
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<b>Cloning:</b>
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The construct, along with homologous flanking sequences corresponding to the faa1 gene locus, were synthesized by iGEM’s DNA synthesis partner IDT.
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The resulting gene fragment was then PCR amplified with the respective primers to attain a higher copy number of the fragment.
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<html><img src="https://static.igem.wiki/teams/4879/wiki/bba-k4879009-pcr-gel.jpg"width="720"height="720"></html>
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The amplified fragment was then gel-extracted and subsequently transformed into our chassis
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:38, 11 October 2023


Yarrowia lipolytica HygR construct

The transcriptional unit for HygR, designed for expression in Y. lipolytica in order to delete the faa1 gene from our chassis.

Characterization

Cloning: The construct, along with homologous flanking sequences corresponding to the faa1 gene locus, were synthesized by iGEM’s DNA synthesis partner IDT. The resulting gene fragment was then PCR amplified with the respective primers to attain a higher copy number of the fragment.

The amplified fragment was then gel-extracted and subsequently transformed into our chassis

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1561
    Illegal suffix found in sequence at 1831
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1825
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
    Illegal NotI site found at 1567
    Illegal NotI site found at 1839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1561
    Illegal EcoRI site found at 1825
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1561
    Illegal suffix found in sequence at 1832
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1561
    Illegal EcoRI site found at 684
    Illegal EcoRI site found at 778
    Illegal EcoRI site found at 1825
    Illegal XbaI site found at 1576
    Illegal SpeI site found at 1832
    Illegal PstI site found at 1846
  • 1000
    COMPATIBLE WITH RFC[1000]