Difference between revisions of "Part:BBa K4800042"
 
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<partinfo>BBa_K4800042 short</partinfo>
 
<partinfo>BBa_K4800042 short</partinfo>
  
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Aspartate transaminase ,from <i>Ruegeria pomeroyi</i> is a pyridoxal-phosphate protein.It catalyzes the formation of glutamate from aspartic acid.It also acts on L-tyrosine,L-phenylalanine and L-tryptophan.  
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We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Spo3471.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:47, 11 October 2023


pTrc99a-Spo3471

We linearized the pTrc99a vector using restriction endonuclease and connected the target gene Spo3471 to it through homologous recombination to obtain the recombinant plasmid pTrc99a-Spo3471.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4045
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4051
    Illegal PstI site found at 2666
    Illegal PstI site found at 4063
    Illegal NgoMIV site found at 3326
    Illegal AgeI site found at 3313
    Illegal AgeI site found at 3437
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4247
    Illegal BsaI site found at 5315
    Illegal SapI.rc site found at 767