Difference between revisions of "Part:BBa K4800040"
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<partinfo>BBa_K4800040 short</partinfo> | <partinfo>BBa_K4800040 short</partinfo> | ||
− | + | We linearized the pTrc99a vector using restriction endonuclease and connected the target gene YahK to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK. | |
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Latest revision as of 08:35, 11 October 2023
pTrc99a-YahK
We linearized the pTrc99a vector using restriction endonuclease and connected the target gene YahK to it through homologous recombination to obtain the recombinant plasmid pTrc99a-YahK.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3627
Illegal PstI site found at 3639 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3639
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3621
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3627
Illegal PstI site found at 3639 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3627
Illegal PstI site found at 3639 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3823
Illegal BsaI site found at 4891
Illegal SapI.rc site found at 767