Difference between revisions of "Part:BBa J435200"
 
Line 14: Line 14:
 
URL: https://freegenes.github.io/genes/BBF10K_000034.html
 
URL: https://freegenes.github.io/genes/BBF10K_000034.html
  
−
<span class='h3bb'><big>'''Electrophoresis of the part by PTSH-Taiwan 2023'''</big></span>
+
<span class='h3bb'><big>'''Documentation of the part by PTSH-Taiwan 2023'''</big></span>
  
−
<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/electrophresis-of-adh1-gfp-tdh3.png" </html>
+
The full-length version of the AHD1 yeast promoter, known for constitutive expression, is notably potent and can drive high levels of gene expression. In contrast, truncated versions of this promoter exhibit constitutive activity but result in relatively low expression levels. The AHD1 promoter is prevalent in various organisms, including human and animal liver, plant cells, and microbial cells, where it serves as a crucial enzyme in the metabolism of short-chain alcohols. Moreover, glucose has been found to enhance the promoter's activity, playing a significant role in numerous physiological processes. In our project, It is used to drive some of the protein in K.Marxianus. Thus, it is also workable in K.Marxianus
−
 
+
−
<center>Figure 1. The Length test.</center>
+
  
  

Latest revision as of 07:49, 11 October 2023


Sc-pAdh1

S. cerevisiae Adh promoter using standard Freegenes syntax

Source organism: S. cerevisiae, Target organism: yeast


Citation: https://www.ncbi.nlm.nih.gov/pubmed/8401308 https://www.ncbi.nlm.nih.gov/pubmed/7737504

ORG: Open Yeast Collection, Open Bioeconomy & The FreeGenes Project

URL: https://freegenes.github.io/genes/BBF10K_000034.html

Documentation of the part by PTSH-Taiwan 2023

The full-length version of the AHD1 yeast promoter, known for constitutive expression, is notably potent and can drive high levels of gene expression. In contrast, truncated versions of this promoter exhibit constitutive activity but result in relatively low expression levels. The AHD1 promoter is prevalent in various organisms, including human and animal liver, plant cells, and microbial cells, where it serves as a crucial enzyme in the metabolism of short-chain alcohols. Moreover, glucose has been found to enhance the promoter's activity, playing a significant role in numerous physiological processes. In our project, It is used to drive some of the protein in K.Marxianus. Thus, it is also workable in K.Marxianus


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]