Difference between revisions of "Part:BBa J435209"
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URL: https://freegenes.github.io/genes/BBF10K_000134.html | URL: https://freegenes.github.io/genes/BBF10K_000134.html | ||
− | <span class='h3bb'><big>''' | + | <span class='h3bb'><big>'''Documentation of the part by PTSH-Taiwan 2023'''</big></span> |
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+ | This is a potent yeast expression promoter derived from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, also known as TDH3 or GAPDH. Research has revealed that the promoter's activity significantly decreases when glycerol or xylose is used as the carbon source or when exposed to high temperatures (42°C). Oxygen conditions have a negligible impact. | ||
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+ | Regarding the protein, the promoter originates from the GAPDH peptide, which plays a role in glycolysis and gluconeogenesis. This protein exists as a tetramer and facilitates the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, primarily located in the cytoplasm and cell wall. Antimicrobial peptides derived from GAPDH are produced by S. cerevisiae and exhibit activity against a broad spectrum of yeast and bacteria commonly found in the context of winemaking. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 07:47, 11 October 2023
Sc-pTDH3
S. cerevisiae TDH3 promoter using standard Freegenes syntax
Source organism: S. cerevisiae, Target organism: yeast
Citation: https://pubmed.ncbi.nlm.nih.gov/7737504/
ORG: Open Yeast Collection, Open Bioeconomy & The FreeGenes Project
URL: https://freegenes.github.io/genes/BBF10K_000134.html
Documentation of the part by PTSH-Taiwan 2023
This is a potent yeast expression promoter derived from the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene, also known as TDH3 or GAPDH. Research has revealed that the promoter's activity significantly decreases when glycerol or xylose is used as the carbon source or when exposed to high temperatures (42°C). Oxygen conditions have a negligible impact.
Regarding the protein, the promoter originates from the GAPDH peptide, which plays a role in glycolysis and gluconeogenesis. This protein exists as a tetramer and facilitates the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, primarily located in the cytoplasm and cell wall. Antimicrobial peptides derived from GAPDH are produced by S. cerevisiae and exhibit activity against a broad spectrum of yeast and bacteria commonly found in the context of winemaking.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]