Difference between revisions of "Part:BBa K4604027"
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<partinfo>BBa_K4604026 short</partinfo>
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<partinfo>BBa_K4604027 short</partinfo>
  
 
piG_K12BSb consists of the LacI promoter, the AdoCbl riboswitch from <i>E. coli K12</i>, the modified T7 RBS, <i>lacI</i> and the trc promoter. The trc promoter can be induced with IPTG. This BioBrick is a sensor for AdoCbl (a bioavailable form of B12) with a variable marker gene.  
 
piG_K12BSb consists of the LacI promoter, the AdoCbl riboswitch from <i>E. coli K12</i>, the modified T7 RBS, <i>lacI</i> and the trc promoter. The trc promoter can be induced with IPTG. This BioBrick is a sensor for AdoCbl (a bioavailable form of B12) with a variable marker gene.  

Revision as of 07:31, 11 October 2023

piG_K12BSb (LacIprom_riboK12_T7RBS_lacI_trcProm_sfGFP)

piG_K12BSb consists of the LacI promoter, the AdoCbl riboswitch from E. coli K12, the modified T7 RBS, lacI and the trc promoter. The trc promoter can be induced with IPTG. This BioBrick is a sensor for AdoCbl (a bioavailable form of B12) with a variable marker gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 215
  • 1000
    COMPATIBLE WITH RFC[1000]