Difference between revisions of "Part:BBa K4785004:Design"
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===Design Notes=== | ===Design Notes=== | ||
The sequence of PslG was truncated to remove the signal peptide that was originally used to mediate the secretion of PslG in P. aeruginosa, so that PSLG could be better expressed in engineered bacteria. | The sequence of PslG was truncated to remove the signal peptide that was originally used to mediate the secretion of PslG in P. aeruginosa, so that PSLG could be better expressed in engineered bacteria. | ||
| − | + | See detailed construction ideas at part:BBa_K4785005 | |
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 07:10, 11 October 2023
pslg
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of PslG was truncated to remove the signal peptide that was originally used to mediate the secretion of PslG in P. aeruginosa, so that PSLG could be better expressed in engineered bacteria. See detailed construction ideas at part:BBa_K4785005
Source
This part is from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)v
References
Yu, S., Su, T., Wu, H. et al. PslG, a self-produced glycosyl hydrolase, triggers biofilm disassembly by disrupting exopolysaccharide matrix. Cell Res 25, 1352–1367 (2015). https://doi.org/10.1038/cr.2015.129