Difference between revisions of "Part:BBa K4785005"

Revision as of 06:28, 11 October 2023

high-mobility group box 1 (hmgb1)

There is a special structure in the biofilm of bacteria: DNABII family DNA-binding proteins twist the extracellular DNA into a Z-type, many Z-types of DNA are connected together to form a three-dimensional grid structure, and polysaccharides act as a "glue" to make the network more stable. Bacteria live in the crevices of these grids, protected from antibiotics and shear forces in the fluid environment.

HMGB1 is a common eukaryotic protein that is a natural component of chromatin and can bind to Z-type DNA. In contact with the reticular structure in the biofilm described above, the protein can compete with DNABII family proteins for DNA binding sites. Due to the different binding ways with DNA, HMGB1 cannot distort DNA as effectively as DNABII family proteins, so the DNA in the biofilm will gradually recover from type Z to type B after binding with HMGB1, resulting in the collapse of the grid structure.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 73

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 <partinfo>BBa_K4785005 short</partinfo>
-A protein from the High Mobility Group-box superfamily is produced by this gene. The nuclear DNA-binding, non-histone protein that is encoded controls transcription and contributes to DNA organization. This protein participates in a number of biological activities, such as cell differentiation, inflammation, and tumor cell migration. This gene has several recognized pseudogenes. Multiple transcript variants that encode the same protein are produced through alternative splicing. Recent studies have found that the structure of HMGB1 is similar to DNABII protein in biofilm, which is responsible for stabilizing eDNA structure. Therefore, HMGB1 can be used to inhibit the growth of biofilm and disrupt mature biofilm. Therefore, our team registered this gene for the convenience of our team and subsequent teams.
+There is a special structure in the biofilm of bacteria: DNABII family DNA-binding proteins twist the extracellular DNA into a Z-type, many Z-types of DNA are connected together to form a three-dimensional grid structure, and polysaccharides act as a "glue" to make the network more stable. Bacteria live in the crevices of these grids, protected from antibiotics and shear forces in the fluid environment.
+HMGB1 is a common eukaryotic protein that is a natural component of chromatin and can bind to Z-type DNA. In contact with the reticular structure in the biofilm described above, the protein can compete with DNABII family proteins for DNA binding sites. Due to the different binding ways with DNA, HMGB1 cannot distort DNA as effectively as DNABII family proteins, so the DNA in the biofilm will gradually recover from type Z to type B after binding with HMGB1, resulting in the collapse of the grid structure.
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