Difference between revisions of "Part:BBa K4879002"
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The direct conversion of free fatty acids (FFAs) into n-alkanes and n-alkenes is an essential feature of our project as it bypasses a series of more expensive and intensive steps in fuel (hydrocarbon) production.
 
The direct conversion of free fatty acids (FFAs) into n-alkanes and n-alkenes is an essential feature of our project as it bypasses a series of more expensive and intensive steps in fuel (hydrocarbon) production.
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<html><img src="https://static.igem.wiki/teams/4879/wiki/bba-k4879002-cvfap.png"width="250"height="250"></html>
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The protein structure of CvFAP, as predicted by AlphaFold
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===Design===
 
===Design===

Revision as of 06:17, 11 October 2023


CvFAP

The fatty acid photodecarboxylase from Chlorella variabilis. [1]

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Usage and Biology

The fatty acid photodecarboxylase, found mainly in the chloroplasts of microalga Chlorella variabilis, catalyses the decarboxylation of free fatty acids into n-alkanes or n-alkenes in the presence of blue light. The reaction is initiated by electron abstraction from the fatty acid to a photo-excited cofactor flavin adenine dinucleotide (FAD).

The direct conversion of free fatty acids (FFAs) into n-alkanes and n-alkenes is an essential feature of our project as it bypasses a series of more expensive and intensive steps in fuel (hydrocarbon) production.


The protein structure of CvFAP, as predicted by AlphaFold


Design

The transcriptional unit for CvFAP (BBa_K4879008) is designed with the coding sequence flanked by the TEF1 promoter (BBa_K3629001) upstream and the XPR2 terminator (BBa_K3629004) downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1424
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 259
    Illegal PstI site found at 691
    Illegal PstI site found at 958
    Illegal NgoMIV site found at 1579
    Illegal AgeI site found at 1555
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Bruder, S., Moldenhauer, E.J., Lemke, R.D. et al. Drop-in biofuel production using fatty acid photodecarboxylase from Chlorella variabilis in the oleaginous yeast Yarrowia lipolytica. Biotechnol Biofuels 12, 202 (2019). https://doi.org/10.1186/s13068-019-1542-4