Difference between revisions of "Part:BBa K4804000"

Revision as of 06:15, 11 October 2023

PETase-R

PETase has PET (poly(ethylene terephtalate) degradation activity first identified in Ideonella sakaiensis. R is the DNA sequence of a repetitive region of PySp1 from the piriform glandular filament of Arachnid. The function of the repeat area is mainly related to the mechanical properties of spider silk fiber.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 304
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 871
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 923
Illegal NgoMIV site found at 965
Illegal AgeI site found at 627
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 <!-- Add more about the biology of this part here
+===Usage and Biology===
 =Introduction=
 We have successfully constructed and tested one new composite part (PETase-R, BBa_K4804000). PETase (PET-digesting enzyme) is secreted by a bacterium (Ideonella sakaiensis 201-F6) that has the ability to degrade PET plastic (Harry P., et al., 2017). Spider silk has high strength and toughness, and has a unique three-dimensional spatial network structure, which can be used as a fixed material for some macromolecules.
@@ Line 61: / Line 63: @@
 [2].Tuo Yi (2019) Research on effects of repeat modules on properties of recombinant spidroins. Master's thesis, Donghua University.
-===Usage and Biology===
 <!-- -->