Difference between revisions of "Part:BBa K4765026"
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===Usage and Biology===
 
===Usage and Biology===
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OpenV3 is a plasmid backbone with AmpR, we constructed all the 'test' parts into it.
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Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. See details in [https://stanford.freegenes.org/products/open-plasmids#genes].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:05, 11 October 2023


OpenV3

contributed by Fudan iGEM 2023

Usage and Biology

Robust, flexible E. coli cloning vector. Designed to be compatible with multiple cloning strategies including restriction ligation, Gibson assembly, SLiCE, and Golden Gate assembly using the FreeGenes MoClo standard. See details in [1].

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 2089