Difference between revisions of "Part:BBa K4630202:Design"

 
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===References===
 
===References===
 +
Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).

Revision as of 05:51, 11 October 2023


pCasop-stgRNA(1+2+3)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 679
    Illegal EcoRI site found at 2517
    Illegal EcoRI site found at 6611
    Illegal XbaI site found at 10720
    Illegal SpeI site found at 671
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 679
    Illegal EcoRI site found at 2517
    Illegal EcoRI site found at 6611
    Illegal NheI site found at 2276
    Illegal NheI site found at 9920
    Illegal SpeI site found at 671
    Illegal NotI site found at 696
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 679
    Illegal EcoRI site found at 2517
    Illegal EcoRI site found at 6611
    Illegal BglII site found at 5378
    Illegal BamHI site found at 1043
    Illegal BamHI site found at 4555
    Illegal BamHI site found at 6545
    Illegal BamHI site found at 12241
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 679
    Illegal EcoRI site found at 2517
    Illegal EcoRI site found at 6611
    Illegal XbaI site found at 10720
    Illegal SpeI site found at 671
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 679
    Illegal EcoRI site found at 2517
    Illegal EcoRI site found at 6611
    Illegal XbaI site found at 10720
    Illegal SpeI site found at 671
    Illegal NgoMIV site found at 9173
    Illegal AgeI site found at 878
    Illegal AgeI site found at 6380
    Illegal AgeI site found at 8044
    Illegal AgeI site found at 8920
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 860
    Illegal SapI site found at 6362


Design Notes

This plasmid is suitable for our project CRISPRrporter. If you need a plasmid that expresses the Cas9 protein and Lambda Red recombinases, please refer to BBa_K4630201


Source

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).

References

Zhao, D., Yuan, S., Xiong, B. et al. Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9. Microb Cell Fact 15, 205 (2016).