Difference between revisions of "Part:BBa K5008000"

Revision as of 04:30, 11 October 2023

CsgA_MBP

The component BBa_K5008000 adds an additional DNA sequence based on BBa_K4161010. This DNA sequence will interact with BBa_K4161010 and give BBa_K4161010 new functions. Specifically, this part is fused with a metal binding protein (MBP) based on CsgA. At the same time, CsgA is a very important subunit of the E. coli peritrichous flagellum and participates in the assembly of the flagellum. Therefore, our part can enable the E. coli peritrichous flagellum to present MBP and play a metal-binding function.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 371
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 371
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 371
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 371
1000
COMPATIBLE WITH RFC[1000]

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 The component BBa_K5008000 adds an additional DNA sequence based on BBa_K4161010. This DNA sequence will interact with BBa_K4161010 and give BBa_K4161010 new functions. Specifically, this part is fused with a metal binding protein (MBP) based on CsgA. At the same time, CsgA is a very important subunit of the E. coli peritrichous flagellum and participates in the assembly of the flagellum. Therefore, our part can enable the E. coli peritrichous flagellum to present MBP and play a metal-binding function.
+<p style="text-align: center!important;">https://static.igem.wiki/teams/5008/wiki/sqpart1.png</p>
 <!-- Add more about the biology of this part here