Difference between revisions of "Part:BBa K4917011"
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| − | <td style = "border: 1px solid black">p207</td><td style = "border: 1px solid black">pRS304</td><td style = "border: 1px solid black">pGAL1_EGFP*siRNAv10_tCYC1</td><td style = "border: 1px solid black">containing GFP sensor fused to siRNA target</td> | + | <td style = "border: 1px solid black">p207</td><td style = "border: 1px solid black">pRS304</td><td style = "border: 1px solid black">pGAL1_EGFP*siRNAv10_tCYC1</td><td style = "border: 1px solid black">Plasmid containing GFP sensor fused to siRNA target</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td style = "border: 1px solid black">p222</td><td style = "border: 1px solid black">Golden Gate assembly</td><td style = "border: 1px solid black"><i>shRNA_v10</i></td><td style = "border: 1px solid black">shRNA expression vectors</td> | <td style = "border: 1px solid black">p222</td><td style = "border: 1px solid black">Golden Gate assembly</td><td style = "border: 1px solid black"><i>shRNA_v10</i></td><td style = "border: 1px solid black">shRNA expression vectors</td> | ||
</tr> | </tr> | ||
| − | + | <tr> | |
| + | <td style = "border: 1px solid black">p224</td><td style = "border: 1px solid black">Golden Gate assembly</td><td style = "border: 1px solid black"><i>pPGK1-AGO1-tPGK1</i> + <i> pTEF1-DCR1-tPGK1</i></td><td style = "border: 1px solid black">Plasmid containing <i>Ago1 and <i>Dcr1</i> transcriptional units</td> | ||
| + | </tr> | ||
</table> | </table> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 04:06, 11 October 2023
shRNA against DWV virus ver10
Starting position for shRNA ver10 in DWV is 2104
Usage and Biology
| Name | Backbone/Plasmids used for GG assembly | Content | Description |
| p207 | pRS304 | pGAL1_EGFP*siRNAv10_tCYC1 | Plasmid containing GFP sensor fused to siRNA target |
| p222 | Golden Gate assembly | shRNA_v10 | shRNA expression vectors |
| p224 | Golden Gate assembly | pPGK1-AGO1-tPGK1 + pTEF1-DCR1-tPGK1 | Plasmid containing Ago1 and <i>Dcr1 transcriptional units |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 66