Difference between revisions of "Part:BBa K4917011"
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===Usage and Biology===
 
===Usage and Biology===
  
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<table style = "border-collapse: collapse">
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    <tr>
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        <td style = "border: 1px solid black"><b>Name&nbsp;&nbsp;</b></td><td style = "border: 1px solid black"><b>Backbone/Plasmids used for GG assembly&nbsp;&nbsp;</b></td><td style = "border: 1px solid black"><b>Content&nbsp;&nbsp;</b></td><td style = "border: 1px solid black"><b>Description&nbsp;&nbsp;</b></td>
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    </tr>
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    <tr>
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        <td style = "border: 1px solid black">p207</td><td style = "border: 1px solid black">pRS304</td><td style = "border: 1px solid black">pGAL1_EGFP*siRNAv10_tCYC1</td><td style = "border: 1px solid black">containing GFP sensor fused to siRNA target</td>
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    </tr>
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    <tr>
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        <td style = "border: 1px solid black">p222</td><td style = "border: 1px solid black">Golden Gate assembly</td><td style = "border: 1px solid black"><i>shRNA_v10</i></td><td style = "border: 1px solid black">shRNA expression vectors</td>
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    </tr>
  
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</table>
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4917011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4917011 SequenceAndFeatures</partinfo>

Revision as of 04:04, 11 October 2023


shRNA against DWV virus ver10

Starting position for shRNA ver10 in DWV is 2104

Usage and Biology

Name  Backbone/Plasmids used for GG assembly  Content  Description  
p207pRS304pGAL1_EGFP*siRNAv10_tCYC1containing GFP sensor fused to siRNA target
p222Golden Gate assemblyshRNA_v10shRNA expression vectors

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 66