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| + | In order to explore the induction conditions of this protein, we set different concentrations of IPTG and analyzed the SDS-PAGE results using software. Finally, we obtained the following table, which shows that there is not much difference in protein expression levels at three different concentrations, but the optimal concentration is 0.5mM. | ||
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Latest revision as of 03:37, 11 October 2023
Contribution of OUC-China
Group: iGEM Team OUC-China 2023
Author: Qingyu Wu
Summary: Exploring the expression conditions of the protein
In order to explore the induction conditions of this protein, we set different concentrations of IPTG and analyzed the SDS-PAGE results using software. Finally, we obtained the following table, which shows that there is not much difference in protein expression levels at three different concentrations, but the optimal concentration is 0.5mM.
Fig 1 Expression intensity of proteins at different induction concentrations
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