Difference between revisions of "Part:BBa M45424:Experience"

 
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=Contribution of OUC-China=
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'''Group''': [https://2023.igem.wiki/ouc-china/parts iGEM Team OUC-China 2023]
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'''Author''': Qingyu Wu
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'''Summary''': Exploring the expression conditions of the protein
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In order to explore the induction conditions of this protein, we set different concentrations of IPTG and analyzed the SDS-PAGE results using software. Finally, we obtained the following table, which shows that there is not much difference in protein expression levels at three different concentrations, but the optimal concentration is 0.5mM.
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<html>
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  <figure>
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      <img src="https://static.igem.wiki/teams/4711/wiki/design/35210630e3590abd1d7c9c85ebd3aaf4.png"width="100%" style="float:center">
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      <figcaption>
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      <p style="font-size:1rem">
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Fig 1 Expression intensity of proteins at different induction concentrations
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      </figcaption>
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  </figure>
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</html>
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Revision as of 03:36, 11 October 2023

Contribution of OUC-China

Group: iGEM Team OUC-China 2023

Author: Qingyu Wu

Summary: Exploring the expression conditions of the protein 

In order to explore the induction conditions of this protein, we set different concentrations of IPTG and analyzed the SDS-PAGE results using software. Finally, we obtained the following table, which shows that there is not much difference in protein expression levels at three different concentrations, but the optimal concentration is 0.5mM.

Fig 1 Expression intensity of proteins at different induction concentrations




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