Difference between revisions of "Part:BBa K4960030"
Line 7: Line 7:
 
Name: pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD<br>
 
Name: pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD<br>
 
Base Pairs: 1824bp<br>
 
Base Pairs: 1824bp<br>
−
Origin:  
+
Origin: <br>
 
Properties: A structural domain that can specifically recognize the EGFR receptor.<br>
 
Properties: A structural domain that can specifically recognize the EGFR receptor.<br>
  

Revision as of 03:14, 11 October 2023

pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD

A flexible linker which can influence the structure of tail fiber connected to the CKGGRAKDC.

Profile

Name: pvc13_NTD-2*GGGSG-E01 DARPin-2*GGGSG -pvc13_CTD
Base Pairs: 1824bp
Origin:
Properties: A structural domain that can specifically recognize the EGFR receptor.

Usage and Biology

Mutant Pvc13-E01-DARPin fused with the DARPin E01 structural domain can enhance the binding ability of PVCs to cells, ultimately enabling targeted delivery of target proteins.[1]

Figure 1.Schematic diagram of the influence of different Linkers on the structure of tail fibers

Figure 2.PVC illustration

Figure 3.Horizontal cutaway view of the fiber region of the syringe.

Special Design

Use the flexible linker on both sides of the CKGGRAKDC to affect the structure and function of the tail protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 309
    Illegal BglII site found at 1674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29.