Difference between revisions of "Part:BBa K4585016"

Latest revision as of 03:06, 11 October 2023

pcDNA3.1(+)-GnRH-C linearized vector

We based on the sequence of the pcDNA3.1(+)-GnRH-C plasmid. Special primers were designed to cut the plasmid into a linear sequence by PCR. The two ends of this linear sequence can be complementary to the GnRH-C homologous recombination fragment to obtain the target product, the pcDNA3.1(+)-GnRH-C plasmid (BBa_K4585011).

1 Diagrams

Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector

2 Caution

The two ends of the pcDNA3.1(+)-GnRH-C linearized vector need to be complementary to the two ends of the corresponding recombinant fragments to ensure the success of homologous recombination ligation.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 249
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 895
Illegal SpeI site found at 249
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 12
Illegal XhoI site found at 901
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 249
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 249
Illegal NgoMIV site found at 1341
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 882

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-                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The agarose gel lectrophoresis of pcDNA3.1(+)-GnRH-C linearized vector</p>
+                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig 1. The agarose gel electrophoresis of pcDNA3.1(+)-GnRH-C linearized vector</p>
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