Difference between revisions of "Part:BBa K4613429"
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Strong constitutive promoter, NAU-CHINA 2023 used this promoter in constructing BBa_K4613462, enabling PAL to have sufficient expression, exerting regulatory functions, and ensuring the normal operation of the system. | Strong constitutive promoter, NAU-CHINA 2023 used this promoter in constructing BBa_K4613462, enabling PAL to have sufficient expression, exerting regulatory functions, and ensuring the normal operation of the system. | ||
| + | ==== Reference ==== | ||
| + | #Bubnov D M, Yuzbashev T V, Khozov A A, et al. Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs[J]. Nucleic Acids Research, 2022, 50(15): 8947–8960. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 20:51, 10 October 2023
PH207
Strong constitutive promoter, NAU-CHINA 2023 used this promoter in constructing BBa_K4613462, enabling PAL to have sufficient expression, exerting regulatory functions, and ensuring the normal operation of the system.
Reference
- Bubnov D M, Yuzbashev T V, Khozov A A, et al. Robust counterselection and advanced λRed recombineering enable markerless chromosomal integration of large heterologous constructs[J]. Nucleic Acids Research, 2022, 50(15): 8947–8960.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 61
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]