Difference between revisions of "Part:BBa K4960004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]<br> | + | Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1,Figure2)<br> |
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| + | Figure1:PVC illustrations<br> | ||
| + | Figure2:Payload illustrations<br> | ||
The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath. | The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath. | ||
Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated. | Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated. | ||
| − | In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12. | + | In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12.(Figure3) |
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| + | Figure3.The protein pvc4 encodes | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 19:38, 10 October 2023
pvc4
Properties
Pvc4 is a part of the sheath.
Usage and Biology
Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices and present in both bacteria and archaea.[1]In structure, PVCs are macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2](Figure1,Figure2)
Figure2:Payload illustrations
The sheath in the pre-contraction state is composed of 23 stacked hexameric rings of sheath proteins, Pvc4, Pvc3, and Pvc2. Among the three, Pvc4 is slightly larger, encircles Pvc5 and forms the first layer of the sheath. Pvc4 is consisted of two major domains (domain I and II). The extra domain of Pvc4, which is smaller than the protrusion domain of Pvc3, lies right below the tail fiber, but no direct interaction is observed. The function of this small protrusion of Pvc4 also remains to be elucidated. In the assembly of the PVC particles, Pvc9 and Pvc4 attach to the initiator tube proteins.to further reinforce the baseplate consisting of Pvc8, Pvc10, Pvc11 and Pvc12.(Figure3)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;