Difference between revisions of "Part:BBa K4724075"

 
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<h2 style="font-weight:600">NusA-LSPET</h2>
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We synthesized the pET-22b(+)-LSPET-12 plasmid from the company and will construct the recombinant plasmid by attaching this fusion tag NusA in front of the LSPETase.
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We fused the NusA fusion tag to the N-terminus of LSPETase to prevent self-aggregation and aggregation by binding and isolating its target protein's prone-to-aggregate folding intermediates, thereby ensuring stable expression of the recombinant protein.
  
 
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Revision as of 19:11, 10 October 2023


NusA-LSPET

We fused the NusA fusion tag to the N-terminus of LSPETase to prevent self-aggregation and aggregation by binding and isolating its target protein's prone-to-aggregate folding intermediates, thereby ensuring stable expression of the recombinant protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1368
    Illegal XhoI site found at 2344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
    Illegal AgeI site found at 1687
  • 1000
    COMPATIBLE WITH RFC[1000]