Difference between revisions of "Part:BBa K4724073:Design"

Revision as of 19:06, 10 October 2023

ompA

Assembly Compatibility:

none

from E.coli

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 ===Design Notes===
-In the original design, because the signal peptide was a small molecule protein with a structural length of 20 to 30 amino acids, so its coding sequence was about 60 to 90 bp, we wanted to design primers to add the coding sequence of the signal peptide to the front of the coding sequence of the ISPET enzyme by PCR. We used the previously designed reverse PCR primers between the RBS and ISPET enzyme sequences to add the sequence of the signal peptide to the primers. The plasmid was subsequently constructed by homologous recombination. After insertion of the signal peptide sequence, we ensured that the signal peptide sequence homologous part was 20 bp on the forward and back primers.
+none
 ===Source===