Difference between revisions of "Part:BBa K4729600"
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Meier, D., Rauch, C., Wagner, M., Klemm, P., Blumenkamp, P., Müller, R., Ellenberger, E., Karia, K. M., Vecchione, S., Serrania, J., Lechner, M., Fritz, G., Goesmann, A., & Becker, A. (2023). A modular cloning (MoClo)-compatible toolbox of ECF sigma factor-based regulatory genetic switches for dual use in Escherichia coli and Sinorhizobium meliloti. In revision.
 
Meier, D., Rauch, C., Wagner, M., Klemm, P., Blumenkamp, P., Müller, R., Ellenberger, E., Karia, K. M., Vecchione, S., Serrania, J., Lechner, M., Fritz, G., Goesmann, A., & Becker, A. (2023). A modular cloning (MoClo)-compatible toolbox of ECF sigma factor-based regulatory genetic switches for dual use in Escherichia coli and Sinorhizobium meliloti. In revision.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4729707 SequenceAndFeatures</partinfo>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 19:03, 10 October 2023


taken from
Meier, D., Rauch, C., Wagner, M., Klemm, P., Blumenkamp, P., Müller, R., Ellenberger, E., Karia, K. M., Vecchione, S., Serrania, J., Lechner, M., Fritz, G., Goesmann, A., & Becker, A. (2023). A modular cloning (MoClo)-compatible toolbox of ECF sigma factor-based regulatory genetic switches for dual use in Escherichia coli and Sinorhizobium meliloti. In revision.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4468
    Illegal PstI site found at 3840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1741
    Illegal BamHI site found at 262
    Illegal BamHI site found at 1917
    Illegal BamHI site found at 2209
    Illegal XhoI site found at 227
    Illegal XhoI site found at 3480
    Illegal XhoI site found at 3834
    Illegal XhoI site found at 7176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3840
    Illegal NgoMIV site found at 111
    Illegal NgoMIV site found at 2054
    Illegal NgoMIV site found at 3894
    Illegal NgoMIV site found at 4119
    Illegal NgoMIV site found at 4224
    Illegal NgoMIV site found at 5254
    Illegal AgeI site found at 5733
  • 1000
    COMPATIBLE WITH RFC[1000]