Difference between revisions of "Part:BBa K4960007"

 
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<partinfo>BBa_K4960007 short</partinfo>
 
<partinfo>BBa_K4960007 short</partinfo>
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===Properties===
  
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Usage and Biology:
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guide the synthesis of the PVC tube initiator.
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]
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<!-- Add more about the biology of this part here-->
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===Usage and Biology===
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Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]<br>
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<figure class="figure">
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc2-3-4-5-6-7.png" class="figure-img img-fluid rounded"  height="200px">
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</figure>
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</html>
 
The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5  through opposite surface charges and electrostatic attraction.In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central  
 
The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5  through opposite surface charges and electrostatic attraction.In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central  
 
spike extending from the inner tube.Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.
 
spike extending from the inner tube.Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.
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<html>
  
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<!-- Add more about the biology of this part here
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<figure class="figure">
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===Usage and Biology===
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<img src="https://static.igem.wiki/teams/4960/wiki/basic-part/pvc-1-5-7.png" class="figure-img img-fluid rounded"  height="200px">
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</figure>
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<partinfo>BBa_K4960007 parameters</partinfo>
 
<partinfo>BBa_K4960007 parameters</partinfo>
 
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===References===
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[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;<br>
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[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;

Revision as of 18:53, 10 October 2023


pvc7

Properties

guide the synthesis of the PVC tube initiator.

Usage and Biology

Photorhabdus virulence cassette (PVC) is one group of extracellular Contractile injection systems (CISs) that are cell-puncturing nanodevices for targeted delivery of proteins .[1]Structurally similar to other CISs, PVCs are also macromolecular complexes containing a rigid tube structure housed in a contractile sheath, which is anchored to a baseplate and sharpened by a spike protein.[2]

The PVC tube linked with payloads consists of three structurally similar tube proteins, such that a complete syringe in a typical length contains 24 stacked hexameric rings of tube proteins, including 2 layers of tube initiators and 22 layers of main tube proteins. Among all related genes,pvc7 produces pvc7 hexameric rings to form the first two initiator rings in the baseplate end of the PVC tube with pvc5 through opposite surface charges and electrostatic attraction.In the meantime,the pvc7 layer (appear to be fusion protein) docks onto the b-barrel ring (pseudo-6-fold) of Pvc8 to help form a continuous central spike extending from the inner tube.Furthermore, pvc7 is encircled by pvc9 and acts as a sheath initiator collectively to prepare for the contraction in the delivery process.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1]Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Programmable protein delivery with a bacterial contractile injection system. Nature. 2023 Apr;
[2]Jiang F, Li N, Wang X, Cheng J, Huang Y, Yang Y, Yang J, Cai B, Wang YP, Jin Q, Gao N. Cryo-EM Structure and Assembly of an Extracellular Contractile Injection System. Cell. 2019 Apr 4;