Difference between revisions of "Part:BBa K4830008:Design"

 
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===Design Notes===
 
===Design Notes===
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The design of FANCF pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
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The design of POAG snp1-3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.
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===Source===
 
===Source===
  
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The original FANCF pegRNA design containing spacer and rttpbs sequence was reported in Prime Editor 2 paper cited in References. With the help of TWIST Bioscience we synthesized those and other necessary sequences, and further combined with 65777 backbone in our lab.
 
  
 
===References===
 
===References===

Revision as of 18:30, 10 October 2023


POAG snp1-3 pegRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of POAG snp1-3 pegRNA includes the U6 promoter, spacer, guide RNA scaffold, rttpbs and polyA tail.

Source

References